PGT-A
Preimplantation Genetic Testing for
Aneuploidy
PGT-A (Preimplantation Genetic Testing for Aneuploidy) is a procedure that allows the determination of the chromosomal state of IVF embryos by screening all 23 pairs of human chromosomes. Only embryos with the correct number of chromosomes will be able to implant and develop into a healthy baby.
Our PGT-A test uses the latest sequencing (NGS) technology to identify those embryos free from chromosome abnormalities, increasing the likelihood of pregnancy per transfer, reducing the risk of miscarriage and allowing confident single embryo transfer.
ENAC | Accreditation Nº 1497/LE2766
In accordance with the requirements of the UNE-EN ISO 15189 standard, for the activities defined in the corresponding technical annex*.
Embryo biopsy at blastocyst stage
Introduction of biopsied cells into the PGT-A tube
Sample shipment at 4ºC
Analysis of the DNA of the biopsied cells
Results in 10 working days
Increases the probability of reproductive success
YOUR PROCESS
Add quality and confidence to your assisted reproduction treatment: go one step further and include the PGT-A® test in your process.
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What is chromosomal aneuploidy?
Abnormalities during early development of the sperm, egg or embryo may lead to an
incorrect number of chromosomes in the embryo. These numerical anomalies in chromosome count are called aneuploidies.
Aneuploidy is responsible for the vast majority of first trimester miscarriages and has been shown to be a major cause of infertility and IVF failure (1).
Chromosomal abnormalities can occur in women of all ages, however the chances are greater with increasing maternal age (2).
Most chromosomal abnormalities are incompatible with life, leading to abortions and the rest of them are associated with genetic disorders like Down syndrome
(resultant form the presence of an extra copy of chromosome 21).
What is the PGT-A?
is a genetic test that allows the determination of the chromosomal state of IVF embryos by screening all 23 pairs of human chromosomes.
PGT-A test is able to identify those embryos free form chromosome abnormalities (euploid embryos) that are more likely to implant and result in a healthy live birth.
By selecting healthy embryos with the right number of chromosomes to be transferred to the uterus, PGT-A:
• Improves IVF success, increasing the likelihood of pregnancy per transfer (3).
• Reduces the risk of miscarriage (4).
• Allows for confident single embryo transfer, reducing the risks and complications associated to multiple pregnancies (5).
• Reduces time to pregnancy by allowing the identification of a normal embryo as soon as possible (6).
• It prevents the birth of babies with genetic diseases of chromosomal origin (7).
Benefits and added value
IGLS uses next-generation sequencing technology to perform PGT-A. The next-generation sequencing platform analyses thousands of DNA sequences specific to each chromosome, allowing the precise identification of gains and losses of up to 10 Mb from specific chromosomes.
This test provides accurate answers to patients, ensuring the transfer of a genetically normal embryo and therefore minimising the incidence of miscarriages and birth defects caused by chromosome number irregularities.
Indications of PGT-A
PGT-A is a beneficial technique for all couples undergoing assisted reproduction treatment, as all pregnancies carry a risk of chromosomal abnormalities, and approximately 50% of the embryos produced in an in vitro fertilisation (IVF) cycle are aneuploid (8).
Aneuploidies are one of the main reasons for difficulty in achieving pregnancy in couples of all ages. However, as a woman ages, the quality of her eggs diminishes, and the risk of producing an embryo with chromosomal abnormalities increases. This is why maternal age is a critical factor for pregnancy success.
Embryo aneuploidy genetic diagnosis increases the likelihood of IVF treatment success in all patients.
It is particularly suitable for helping couples with recurrent miscarriage, couples with previous IVF failure, women of advanced maternal age (over 35 years), couples with a family history of chromosomal issues, couples opting for the transfer of a single embryo with assured success, and patients who want to avoid the futile undertaking of future transfers of cryopreserved embryos.
1. Hassold T. & Hunt P. – Nat Rev Genet 2001; 2: 280-291.
2. Fragouli E. et al. – Hum Genet 2013; 132(9): 1001-13.
3. Scott R.T. et al. – Fertil Steril 2013; 100: 697-703.
4. Grifo J.A. et al. – J Assist Reprod Genet 2013; 30(2): 259-264.
5. Yang Z. et al. – Mol Cytogenet 2012; 5: 24.
6. Sermon K. et al. – Mol Hum Reprod 2016; 22(8): 845-57.
7. Fiorentino F. et al. – Hum Reprod 2011; 26(7): 1925-35.
8. Ata B. et al. – Reprod Biomed Online 2012; 24(6): 614-20.